Isolation and Identification of Candida spp. from Mastitis Cattle Milk and Determination of Antifungal Susceptibilities

Isolation and identification of Candida spp. and determination of their antifungal susceptibilities that cause mycotic mastitis in cattle in Tekirdag province and its counties in Marmara Region were aimed in this research. 100 mastitis milk samples were used in the study. Microorganisms isolated as Candida spp. were identified by API 20C AUX rapid identification kit. Antifungal susceptibility rates of identified Candida spp. were determined by using disc diffusion technique. Candida spp. was detected in the 25 of the 100 mastitis milk samples. According to results of API 20C AUX rapid identification kit, 8 C. krusei (32%), 5 C. albicans (20%), 3 of each C. boidinii, C. famata and C. kefyr (12%), 2 C. spherica (8%) and 1 C. thermophilia (4%) were identified from the 25 isolated strains. In the evaluation of test results of isolated strains that performed in respect to M-44 directive of CLSI (Method for Antifungal Disc Diffusion Susceptibility Testing of Yeasts; Approved Guideline); all strains were found 100% susceptible to Ketoconazole and 100% resistant to Fluconazole, Miconazole, Amphoteracine B and Flucytosine. Two of the C. albicans (40%) and 1 of the C. krusei (12.5%) isolates were susceptible Nystatin while all the other isolates were moderately resistant to that. This study contributes significantly to the literature as one of the rare studies addressing the development of antifungal resistance.